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. 2017 May 15;31(10):1054–1065. doi: 10.1101/gad.297424.117

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© 2017 Anzo et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — DA1-PN dendritic Eph recognizes adjacent PN Ephrin as a ligand. (A) Drawings showing the hypothesis that DA1 PN dendrites recognize Ephrin on the dendrites of adjacent glomeruli to stay in DA1. The behavior of wild-type DA1 PN dendrites was analyzed when ephrin was knocked down in surrounding ad-PNs such as VA1d PNs. (B–E) Wild-type DA1 l-PNs and VA1d ad-PNs were labeled by mtdTomato-HA and mCD8-GFP, respectively. n = 7. The proper dendritic boundary was formed between DA1 l-PNs stained with anti-HA antibody (C) and VA1d ad-PNs stained with anti-GFP antibody (D). (F–I) Wild-type DA1 l-PNs and ephrin-shRNA-expressing VA1d ad-PNs were labeled simultaneously. n = 7. (G) Wild-type DA1 l-PNs spilled over to the adjacent glomeruli such as the VA1d glomerulus in 100% penetrance (arrow; seven out of seven). (H) ephrin-shRNA expression in VA1d ad-PNs cell-autonomously caused dendritic spillover into DA1 (arrowhead). Blue represents DN-cadherin staining. Bar, 25 µm.