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hESC-derived retinal organoids at (A, D) 46 DIC, (B, E) 89 DIC, and (C, F) 151 DIC coimmunostained for retinal progenitor cell marker VSX2 (CHX10), photoreceptor marker CRX, and ganglion cell marker BRN3. (C', F') Immunostaining of 146 DIC organoids, an age similar to organoids in (C, F), respectively, that did not undergo prior live imaging. Scale bars: 100 μm.
