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. 2017 Jul;58(9):3311–3318. doi: 10.1167/iovs.16-20796

Figure 6.

Figure 6

HSpec of hESC-derived retinal organoids at (A, D, G, J) 46 DIC, (B, E, H, K) 89 DIC, and (C, F, I, L) 151 DIC. (A–C) Autofluorescence of organoid cross section. (D–L) Calculated distributions of spectral signatures of free NADH (yellow, D–F), retinol (dark green, G–I) and retinoic acid (red, J–L). White boxes, regions sampled for retinol and retinoic acid signal. (M, N) retinol and retinoic acid signals at deep and superficial positions in four organoids imaged at each age. Data points and error bars indicate mean and standard deviation. Scale bars: 50 μm.