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. Author manuscript; available in PMC: 2017 Jul 3.
Published in final edited form as: Mol Nutr Food Res. 2016 May 17;60(6):1427–1436. doi: 10.1002/mnfr.201500918

Figure 2. miR-194 suppresses PC3 cells invasiveness.

Figure 2

Matrigel Transwell cell invasion assay was performed to test the effect of miR-194 on the invasive ability of PC3 cells. PC3 cells were treated with miR-194 mimic, 2.5μM PEITC, and a combination of miR-194 inhibitor and 2.5μM PEITC prior to seeding in the chamber. The cells that invaded the basal side of the membrane were captured under microscopy and the cell numbers were counted. A) representative imagines of the Transwell assays for cell migration; B) invasion ability of PC cells quantified by counting the number of cells that invaded the underside of the membrane; C) validation of transfection efficiency of miR-194 mimic and miR-194 inhibitor by qPCR. Data are presented as the mean ± SD from at least three independent experiments.