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. 2017 Jul 3;12(7):e0179884. doi: 10.1371/journal.pone.0179884

Fig 4. Silencing of mutant p53 in MiaPaCa-2 cells stimulates SAHA-dependent decrease and increase in cell viability and cell death, respectively.

Fig 4

(A) Phase-contrast micrographs. MiaPaCa-2 cells were transfected with control siRNA or with siRNA against p53, and then treated with DMSO, 1 μM of SAHA or left untreated. Forty-eight hours after treatment, the representative pictures were taken. (B) WST assay. MiaPaCa-2 cells were transfected with control siRNA or with siRNA against p53, and treated with DMSO or with the indicated concentrations of SAHA. Forty-eight hours after SAHA exposure, cells were analyzed by the standard WST cell survival assay. Solid and grey boxes indicate control siRNA- and p53 siRNA-transfected cells, respectively. (C) FACS analysis. MiaPaCa-2 cells were transfected with control siRNA or with siRNA against p53, and treated with DMSO or with the indicated concentrations of SAHA. Forty-eight hours after treatment, floating and adherent cells were harvested and subjected to flow cytometric analysis. Solid and grey boxes indicate control siRNA- and p53 siRNA-transfected cells, respectively.