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. 2017 Jul 3;12(7):e0179884. doi: 10.1371/journal.pone.0179884

Fig 5. Forced depletion of mutant p53 augments SAHA-mediated accumulation of TAp63 and reduction of RUNX2.

Fig 5

MiaPaCa-2 cells were transfected and treated as in Fig 4A. Forty-eight hours post-treatment, cell lysates and total RNA were prepared and analyzed by immunoblotting (A) and RT-PCR (B), respectively. Actin and GAPDH were used as a loading and an internal control, respectively.