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. 2017 Jul 3;12(7):e0179884. doi: 10.1371/journal.pone.0179884

Fig 6. Knockdown of RUNX2 potentiates SAHA-dependent cell death of MiaPaCa-2 cells.

Fig 6

(A) Representative photos. MiaPaCa-2 cells were transfected with control siRNA or with siRNA targeting RUNX2. Twenty-four hours after transfection, cells were treated with DMSO, 1 μM of SAHA or left untreated. Forty-eight hours after treatment, the representative pictures were taken. (B) Cell survival assay. MiaPaCa-2 cells were transfected with control siRNA or with siRNA targeting RUNX2, and treated with DMSO or with the indicated concentrations of SAHA. Forty-eight hours after treatment, cells were analyzed by WST cell survival assay. Solid and grey boxes indicate control siRNA- and RUNX2 siRNA-transfected cells, respectively. (C) FACS analysis. MiaPaCa-2 cells were transfected with control siRNA or with siRNA targeting RUNX2, and treated with DMSO or with the indicated concentrations of SAHA. Forty-eight hours after treatment, floating and adherent cells were harvested and subjected to flow cytometric analysis. Solid and grey boxes indicate control siRNA- and RUNX2 siRNA-transfected cells, respectively.