Fig 7. Silencing of RUNX2 stimulates SAHA-induced accumulation of TAp63 and reduction of mutant p53.

MiaPaCa-2 cells were transfected and treated as in Fig 6A. Forty-eight hours after treatment, cell lysates and total RNA were extracted and processed for immunoblotting (A) and RT-PCR (B), respectively. Actin and GAPDH were used as a loading and an internal control, respectively.