Table S1.

Sequences of dsDNA targets used in degradation and binding assays

Oligonucleotide no.	Sequence
1	GCTGTACGTCACTATCGAAGCAATACAGGTAGACGCGGACATCAAGCCCGCCGTGAAGGTGCAGCTTCTCTACAGAGTGC
2	GCACTCTGTAGAGAAGCTGCACCTTCACGGCGGGCTTGATGTCCGCGTCTACCTGTATTGCTTCGATAGTGACGTACAGC
3	GCTGTACGTCACTATCGAAGCAATACAGGTAGACGCGGACATCAAGCCCGCCGTGAAGGTGCAGCTTCTCTACAGAGTGC
4	GCACTCTGTAGAGAAGCTGCACCAAGTGCCGCCGCTTGATGTCCGCGTCTACCTGTATTGCTTCGATAGTGACGTACAGC
5	AGCGACTCCCGAGCAATCACTGTTGGCAAGCCAGGATCTGAACAATACCGTCTTGCTTTCGGCTTGCCGCGC
6	GCGCGGCAAGCCGAAAGCAAGACGGTATTGTTCAGATCCTGGCTTGCCAACAGTGATTGCTCGGGAGTCGCT

Oligonucleotide sequences are shown 5′–3′. Protospacers (underlined) and PAMs (bold) are indicated on the complementary strands only. (1 and 2) Sequence used to make duplexed dsDNA substrates for I-F experiments. (3 and 4) Sequence used to make dsDNA substrates with internal 10-nt bubble for I-F experiments. (5 and 6) Sequence used to make duplexed dsDNA substrates for I-E experiments.