Fig. S1.

Identification of in vivo phosphorylation sites of CaMKIIβ in Purkinje cells. (A) Mass spectrometric sequence coverage of purified CaMKIIβ from mice Purkinje cells. To identify the phosphorylation sites of CaMKIIβ in Purkinje cells, we immunoprecipitated CaMKIIα from two mice cerebella using anti-CaMKIIα antibody and purified CaMKIIβ in Purkinje cells as an immunocomplex of CaMKIIα. Then, the immunocomplex was subjected to mass-spectrometric phosphopeptide mapping. Detected peptides are highlighted in yellow. Amino acid sequence was from accession no. NP_031621.3 (Mus musculus). (B) Amino acid sequences of phosphopeptides of CaMKIIβ. Phosphorylation sites in phosphopeptides are indicated by asterisks.