Fig. S3.

The kinase activity does not affect S315 phosphorylation-dependent interaction of CaMKIIβ with F-actin. (A) Immunostaining of K43M, K43M/S315D, and K43M/S315A mutant of HA-CaMKIIβ in HeLa cells. F-actin was visualized with phalloidin-594. (Scale bar, 20 μm.) (B) Colocalization of each mutant form of CaMKIIβ and F-actin (phalloidin-594) was examined as in Fig. 5C. ***P < 0.0001, one-way ANOVA with Bonferroni’s test for multiple comparisons. The numbers of cells are indicated in the graph.