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Verification of RNA-Seq analysis of prodomain-treated TA muscles. (A) qRT-PCR analysis was used to assess mRNA expression of Actc1, Klhl38, and Igfn1 in TA muscles of WT mice treated with activin B and/or myostatin prodomains. (B) The effect of Smad6 on prodomain-induced changes in the mRNA expression of Actc1, Klhl38, and Igfn1 in TA muscles of WT mice (n = 5, one-way ANOVA with Tukey’s post hoc test, data groups with different letters achieved significance of P < 0.05).
