Fig. 3.
Hm1a prevents frequency-dependent current reduction in available channels. (A) Representative current traces from oocytes expressing Nav1.1 before stimulation (control, gray trace), and after 50-Hz simulated action potentials for 1 s at a holding potential (HP) of −90 mV in the absence (black trace) or presence (red trace) of 100 nM Hm1a. (B) Group data showing the availability of Nav1.1 following the simulated action potentials at the rate and holding potential indicated. Hm1a significantly increases Nav1.1 availability over a range of conditions. Values are shown as mean ± SEM with n = 5. P < 0.001, two-way ANOVA.