Fig. S1.
Effect of LqqIV on Nav1.1 SSI and voltage-dependence of slow inactivation. (A) Normalized steady-state inactivation (I/Imax, open circles) relationships of Nav1.1 before (black) and after (red) treatment with 100 nM LqqIV, measured at +40 mV in low external Na+. The SSI relationship was obtained using the protocol shown in Fig. 1D. Also shown is the SSI under normal saline conditions (black filled circles). (B) Voltage dependence of slow inactivation in the absence (black) or presence (red) of 100 nM LqqIV (open circles). Oocytes were held at −90 mV and exposed to the different conditioning voltages for 10 s, followed by a −100-mV pulse for 50 ms to remove fast inactivation and then a test pulse to −20 mV. Values are shown as mean ± SEM with n = 5. Boltzmann fit values are reported in the text.