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Fine mapping of the NFS1 locus. (A) The 1:2:1 segregation ratio of the Fix+, Fix+/−, and Fix− phenotypes in the near-isogenic progeny derived by selfing of the residual heterozygous RHL-NFS1 plants, suggesting a single gene control of the trait. (B) The NFS1 locus was delimited to a 4.9-kb genomic region between markers SNP5 and SNP7. Numbers indicate the number of recombination breakpoints separating the marker from NFS1 based on the mapping of 10,221 progeny.
