Fig. S5.

CRISPR/Cas9-mediated knockout of the DZA315 alleles of NCR-α or NCR-β in the NFS1^+/+ background of RHL-NFS1. Knockout of NCR-α (A) or NCR-β (B) retained the Fix⁺ phenotype. Inset figures showed GUS staining of the same nodules/roots in the main panel, as an indicator for the transgenic roots. (Scale bars: 1 mm.) (C) The transgenic root that formed Fix⁺ nodules in A contained three heterogeneous mutant alleles: one with an “A” insertion, one with a “G” insertion, and another allele with a 5-bp deletion. (D) The transgenic root that formed Fix⁺ nodules in B contained three heterogeneous mutant alleles: one with a “T” insertion, one with a “G” insertion, and another allele with a 1-bp deletion.