Fig. S10.

The NFS1⁻ peptide colocalizes with bacteroids in transgenic M. truncatula nodules (Fix⁻). (A) NFS1⁻/mCHERRY primarily localizes to the cells beyond the infection zone. (B) GFP-tagged bacteria are mostly restricted to the infection zone and the transition zone. (C) An overlay of A and B showing that the presence of the peptide and the bacteria is mostly mutually exclusive. The box indicates a cell layer in the transition zone that shows colocalization of the peptide with the bacteroids. (Scale bars: 50 µm.)