Table S1.
Genotyping the NFS1 and NFS2 loci in the RIL population derived from the cross between A17 and DZA315
| RIL no. | Genotype | Phenotype | RIL no. | Genotype | Phenotype | RIL no. | Genotype | Phenotype | |||
| NFS1 | NFS2 | NFS1 | NFS2 | NFS1 | NFS2 | ||||||
| 1 | −/− | −/− | Fix− | 240 | −/− | −/− | Fix− | 98* | −/− | +/+ | Fix+ |
| 3 | −/− | −/− | Fix− | 241 | −/− | −/− | Fix− | 101 | +/+ | +/+ | Fix+ |
| 8 | −/− | +/+ | Fix− | 243 | −/− | −/− | Fix− | 108 | +/+ | +/+ | Fix+ |
| 12 | −/− | −/− | Fix− | 2 | +/+ | +/+ | Fix+ | 109 | +/+ | +/+ | Fix+ |
| 15 | −/− | NA | Fix− | 4* | +/+ | −/− | Fix+ | 110 | +/+ | +/+ | Fix+ |
| 20 | −/− | +/+ | Fix− | 5 | +/+ | +/+ | Fix+ | 111 | +/+ | +/+ | Fix+ |
| 26 | −/− | +/+ | Fix− | 6 | +/+ | +/+ | Fix+ | 113 | +/+ | +/+ | Fix+ |
| 29 | −/− | −/− | Fix− | 7 | +/+ | −/− | Fix+ | 116 | +/+ | +/+ | Fix+ |
| 30 | −/− | +/+ | Fix− | 9 | +/+ | +/+ | Fix+ | 128 | +/+ | +/+ | Fix+ |
| 32 | −/− | −/− | Fix− | 10 | +/+ | +/+ | Fix+ | 131 | +/+ | +/+ | Fix+ |
| 43 | −/− | −/− | Fix− | 11* | +/+ | −/− | Fix+ | 135* | −/− | +/+ | Fix+ |
| 44 | −/− | −/− | Fix− | 13* | −/− | −/− | Fix+ | 136 | +/+ | +/+ | Fix+ |
| 48 | −/− | +/+ | Fix− | 14* | −/− | −/− | Fix+ | 142* | −/− | +/+ | Fix+ |
| 49 | −/− | −/− | Fix− | 17 | +/+ | +/+ | Fix+ | 144* | −/− | +/+ | Fix+ |
| 50 | −/− | +/+ | Fix− | 18 | +/+ | +/+ | Fix+ | 148 | +/+ | +/+ | Fix+ |
| 53 | −/− | −/− | Fix− | 19 | +/+ | NA | Fix+ | 154 | +/+ | +/+ | Fix+ |
| 56 | −/− | −/− | Fix− | 21 | +/+ | +/+ | Fix+ | 157* | +/+ | −/− | Fix+ |
| 60 | −/− | −/− | Fix− | 23 | +/+ | +/+ | Fix+ | 159 | +/+ | +/+ | Fix+ |
| 67 | −/− | +/+ | Fix− | 25* | +/+ | −/− | Fix+ | 162 | +/+ | +/+ | Fix+ |
| 69 | −/− | −/− | Fix− | 27 | +/+ | +/+ | Fix+ | 166 | +/+ | +/+ | Fix+ |
| 70 | −/− | −/− | Fix− | 33* | +/+ | −/− | Fix+ | 169 | +/+ | +/+ | Fix+ |
| 79 | −/− | −/− | Fix− | 35 | +/+ | +/+ | Fix+ | 186 | +/+ | +/+ | Fix+ |
| 80 | −/− | −/− | Fix− | 36* | +/+ | −/− | Fix+ | 188 | +/+ | +/+ | Fix+ |
| 81 | −/− | +/+ | Fix− | 39 | +/+ | +/+ | Fix+ | 190* | +/+ | −/− | Fix+ |
| 93 | −/− | −/− | Fix− | 40 | +/+ | +/+ | Fix+ | 193* | +/+ | −/− | Fix+ |
| 96 | −/− | −/− | Fix− | 45 | +/+ | +/+ | Fix+ | 196* | −/− | +/+ | Fix+ |
| 100 | −/− | −/− | Fix− | 46 | +/+ | +/+ | Fix+ | 197* | +/+ | −/− | Fix+ |
| 102 | −/− | −/− | Fix− | 51 | +/+ | +/+ | Fix+ | 200 | +/+ | +/+ | Fix+ |
| 127 | −/− | +/+ | Fix− | 54 | +/+ | +/+ | Fix+ | 201 | +/+ | NA | Fix+ |
| 147 | −/− | −/− | Fix− | 57* | +/+ | −/− | Fix+ | 207 | +/+ | +/+ | Fix+ |
| 152 | −/− | −/− | Fix− | 58* | −/− | −/− | Fix+ | 213* | −/− | +/+ | Fix+ |
| 164 | −/− | −/− | Fix− | 59* | +/+ | −/− | Fix+ | 215* | +/+ | −/− | Fix+ |
| 180 | −/− | −/− | Fix− | 61* | −/− | +/+ | Fix+ | 217* | +/+ | −/− | Fix+ |
| 182 | −/− | +/+ | Fix− | 62 | +/+ | +/+ | Fix+ | 219 | +/+ | +/+ | Fix+ |
| 184 | −/− | −/− | Fix− | 64 | +/+ | +/+ | Fix+ | 220 | +/+ | NA | Fix+ |
| 187 | −/− | −/− | Fix− | 65 | +/+ | +/+ | Fix+ | 222 | +/+ | +/+ | Fix+ |
| 189 | −/− | −/− | Fix− | 66 | +/+ | +/+ | Fix+ | 224 | +/+ | +/+ | Fix+ |
| 191 | −/− | −/− | Fix− | 73 | +/+ | +/+ | Fix+ | 229 | +/+ | +/+ | Fix+ |
| 194 | −/− | −/− | Fix− | 75 | +/+ | +/+ | Fix+ | 231 | +/+ | +/+ | Fix+ |
| 203 | −/− | −/− | Fix− | 77 | +/+ | +/+ | Fix+ | 232 | +/+ | +/+ | Fix+ |
| 205 | −/− | +/+ | Fix− | 78 | +/+ | +/+ | Fix+ | 234 | +/+ | +/+ | Fix+ |
| 206 | −/− | +/+ | Fix− | 83* | +/+ | −/− | Fix+ | 236* | −/− | −/− | Fix+ |
| 208 | −/− | −/− | Fix− | 84* | −/− | +/+ | Fix+ | 238 | +/+ | +/+ | Fix+ |
| 209 | −/− | −/− | Fix− | 86 | +/+ | +/+ | Fix+ | 239* | −/− | +/+ | Fix+ |
| 212 | −/− | −/− | Fix− | 87* | +/+ | −/− | Fix+ | 244* | +/+ | −/− | Fix+ |
| 225 | −/− | −/− | Fix− | 88 | +/+ | +/+ | Fix+ | 246 | +/+ | +/+ | Fix+ |
| 226 | −/− | −/− | Fix− | 92 | +/+ | +/+ | Fix+ | RHL−NFS1 | +/− | +/+ | Segregation |
| 230 | −/− | −/− | Fix− | 95 | +/+ | +/+ | Fix+ | RHL−NFS2* | −/− | +/− | Segregation |
| 235 | −/− | +/+ | Fix− | 97 | +/+ | NA | Fix+ | ||||
−/−, homozygous A17 alleles; +/+, homozygous DZA315 alleles; +/−, heterozygous alleles; NA, not available due to a lack of DNA or seeds when genotyping the NFS2 locus.
Indicates the lines that carry NFS1−/− and/or NFS2−/− genotypes but show the Fix+ phenotype or phenotypic segregation.