Fig. 2.
SMCs synergize with M1 to potentiate the bystander killing effect. (A) HCT 116 cells were treated with recombinant M1 (rM1)-iRFP (MOI = 1 pfu/cell) with or without 5 μM LCL161 for 72 h and stained with Hoechst 33342. (Scale bars: 20 μm.) (B) Percentages of caspase-3/7+/− and iRFP+/− cells were detected using flow cytometry in HCT 116 cells treated as in A. (C) Effect of UV irradiation (1 h) on viability (TCID50 method) of M1 in the supernatants. Supernatants were collected from HCT 116 and Huh-7 cells (MOI = 1 pfu/cell) infected with M1 for 48 h. (D and E) Ten percent of supernatants inactivated by UV irradiation in C were treated with or without boiling and were then combined with LCL161, after which cell viability was detected. Error bars represent mean ± SD obtained from three independent experiments. N.D., not detected; n.s., no significance; TCID50, median tissue culture infectious dose. *P < 0.05; ***P < 0.001.