Fig. 4.

c-IAP1 and c-IAP2 play key roles in the enhanced oncolytic effect induced by SMCs. The effect of LCL161 on expression of three classical IAPs in HCT 116 cells (A) and Huh-7 (B) cells is shown. The effect of siRNAs to c-IAP1 (C), c-IAP2 (D), and XIAP (E) on the protein level of each is shown. (F–H) HCT 116 cells were treated with siRNAs to cIAP1, cIAP2, and XIAP. Forty-eight hours later, M1 virus (MOI = 1 pfu/cell) was added for another 48 h and cell viability was detected. Error bars represent mean ± SD obtained from three independent experiments. L, LCL161; M, mock; NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001.