Fig. 5.

LCL161 increases replication of M1 virus. The effect of LCL161 on replication of M1 at protein (MOI = 1 pfu/cell) (A), mRNA (MOI = 1 pfu/cell, 24 h postinfection) (B) and viral titer (C) levels in HCT 116 cells is shown. Viral titer (MOI = 0.1 pfu/cell) was detected with the TCID₅₀ method. (D) Effect of siRNAs to c-IAP1, c-IAP2, and XIAP on replication of M1 (MOI = 1 pfu/cell, 48 h postinfection) in HCT 116 cells. Viral titer was detected with the TCID₅₀ method. The viral yield of each treatment was compared with the NC group. Error bars represent mean ± SD obtained from three independent experiments. LM, LCL161 + M1. *P < 0.05; ***P < 0.001.