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SMCs synergize with M1 to potentiate the bystander killing effect in Huh7 cells. (A) Huh-7 cells were treated with recombinant M1 (rM1)-iRFP (MOI = 1 pfu/cell) with or without 5 μM LCL161 for 72 h, and stained with Hoechst 33342. (Scale bars: 20 μm.) (B) Percentages of caspase-3/7+/− and iRFP+/− cells were detected using flow cytometry in Huh-7 cells treated as in A. Error bars represent mean ± SD obtained from three independent experiments. LM, LCL161 + M1.
