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. 2017 Jun 12;114(26):6812–6817. doi: 10.1073/pnas.1701002114

Fig. S3.

Fig. S3.

Roles of IL-8, IL-1A, and TRAIL in Huh-7 cells and other cytokines cannot synergize with LCL161 to induce cell death. (A) Huh7 cells were treated with LCL161 or LCL161 plus different concentrations of recombinant proteins of IL-8, IL-1A, and TRAIL for 72 h, and cell viability was detected. (B and C) HCT 116 and Huh-7 cells were treated with LCL161 or LCL161 plus different concentrations of recombinant proteins of CXCL-1, CXCL-2, IFN-κ, IL-28, or IL-29 for 72 h, and cell viability was detected. Huh-7 cells were treated with M1 for different lengths of time, and the mRNA level (D) and protein level (E) of IL-8, IL-1A, and TRAIL were detected with RT-quantitative PCR and ELISA. (F) HCT 116 and Huh-7 cells were treated with different concentrations of TRAIL or TRAIL plus M1 (MOI = 1 pfu/cell), and cell viability was detected. Error bars represent mean ± SD obtained from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.