Fig. S5.

LCL161 increases replication of M1 virus in Huh-7, but not normal, cells. (A and B) Effect of LCL161 on replication of M1 virus at protein (MOI = 1 pfu/cell) and viral titer levels in Huh-7 cells. Viral titer (MOI = 0.1 pfu/cell) was detected with the TCID₅₀ method. (C) Effect of siRNAs to c-IAP1, c-IAP2, and XIAP on the replication of M1 (MOI = 1 pfu/cell, 48 h postinfection) in Huh-7 cells. Viral titer was detected with the TCID₅₀ method. The viral yield of each treatment was compared with the NC group. The effect of siRNAs to c-IAP1, c-IAP2, and XIAP on expression of viral protein E1 (MOI = 1 pfu/cell, 48 h postinfection) in HCT 116 (D–F) and Huh-7 (G–I) cells is shown. (J) NCM460 and L-02 cells were treated with M1 or M1 plus LCL161 for 24 h and 48 h. Western blot of E1 and NS3 is shown. Error bars represent mean ± SD obtained from three independent experiments. P, positive control from HCT 116 cell lysate infected with M1 virus; L, LCL161; TCID₅₀, median tissue culture infectious dose. *P < 0.05; **P < 0.01; ***P < 0.001.