Fig. S7.

Combination of LCL161 and M1 inhibits tumor progression in a Huh-7 mouse xenograft model. (A–C) Effect of LCL161 and M1 virus combination on the mouse xenograft model with a Huh-7 tumor. (n = 5, tumor volume in each group was compared with the relative control group). (D) Intratumoral expression of Ki67 and cleaved caspase-3 (Cl-casp3) was detected by immunological and histological chemistry in Huh-7 tumors. (Scale bars: 50 μm.) (E) Quantitation of Ki67-positive and Cl-casp3–positive cells from multiple Huh-7 tumors; relative positive cells are shown (n = 5). (F) HCT 116 cells were implanted s.c. in 4-wk-old female BALB/c nude mice. Eight days later, the mice were treated once with M1 virus (1 × 10⁷ pfu, i.v.) or M1 plus LCL161 (20 mg/kg, i.p.). Forty-eight hours later, tumors were stripped out and homogenated for M1 viral load detection by the TCID₅₀ method (n = 7). Error bars represent mean ± SD. D, day; L+M, LCL161 + M1. *P < 0.05; ***P < 0.001.