The role of Vg and viral CP in transovarial transmission of virus. (A) Vg mRNA levels after feeding with dsRNAs. (B and C) dsVg treatment delayed whitefly ovary development (B) and reduced egg production (C) of whitefly. For B, sample sizes were 30 females per treatment, and for C, nine replicates per treatment (five females/replicate). (D and E) TYLCV DNA levels in whole bodies (D) and ovaries (E) of whiteflies after feeding with dsRNAs. (F) dsVg treatment reduced transovarial transmission of TYLCV to eggs. (G) Oral ingestion of anti-Vg antibody decreased transmission rate of TYLCV to eggs. (H) The viral DNA level of mutant TYLCV (mTYLCV) and mutant PaLCuCNV (mPaL) in whitefly whole bodies. (I) Transovarial transmission rate of mTYLCV and mPaL via ovum by MEAM1 whitefly. We detected 45–80 eggs for F, G, and I. (A–I) Mean ± SEM of three independent experiments. P < 0.05 (one-way ANOVA, LSD test).