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. 2017 Jun 12;114(26):6746–6751. doi: 10.1073/pnas.1701720114

Fig. S6.

Fig. S6.

Localization of viruses and Vg in different tissues of MED whiteflies. (A–D) Localization of TYLCV or PaLCuCNV (PaL) in midguts (MGs) (n = 24), primary salivary glands (PSGs) (n = 24), and ovaries (n = 24) of viruliferous MED whiteflies at 11 DAE. For A, TYLCV and PaL virions were detected by use of a mouse anti-CP monoclonal antibody and goat anti-mouse IgG labeled with Dylight 488 (green) secondary antibody. White arrows indicate mature oocytes. For B–D, mean ± SEM from three experiments. P < 0.05 (one-way ANOVA, LSD test). (E) TYLCV and Vg colocalized with each other in ovariole of MED whitefly. TYLCV virions were detected by use of a rabbit anti-CP polyclonal antibody and goat anti-rabbit IgG labeled with Dylight 488 (green) secondary antibody. Vg was detected by use of a mouse anti-Vg monoclonal antibody and goat anti-mouse IgG labeled with Dylight 549 (red) secondary antibody. Cell nucleus was stained with DAPI (blue). Yellow color indicates the overlay of red and green.