Table S4.
Primers used in this study
| Primer name* | Sequence, 5′–3′† | Length, bp | Purpose |
| TYLCV-F | CAGAGCAGTTGATCATG | 682 | TYLCV DNA detection |
| TYLCV-R | ATCGAAGCCCTGATATCCCCCGTGG | ||
| PaLCuCNV-F | TAGTCATTTCCACTCCCGC | 645 | PaLCuCNV DNA detection |
| PaLCuCNV-R | TGATTGTCATACTTCGCAGC | ||
| mTYLCV-F | CAGAGCAGTTGATCATG | 679 | Mutant TYLCV DNA detection |
| mTYLCV-R | ATCGAAGCCCTGATATCCCCCGTGG | ||
| mPaLCuCNV-F | CCTGATGTTCCTCGTGGTT | 408 | Mutant PaLCuCNV DNA detection |
| mPaLCuCNV-R | CCTGTTCCTTCATTCCAGAG | ||
| QTYLCV(mTYLCV)-F | GAAGCGACCAGGCGATATAA | 189 | qPCR for TYLCV (mutant TYLCV) |
| QTYLCV(mTYLCV)-R | GGAACATCAGGGCTTCGATA | ||
| QPaL(mPaL)-F | GACCCGCCGATATAGTCATT | 109 | qPCR for PaLCuCNV (mutant PaLCuCNV) |
| QPaL(mPaL)-R | GTTTGTGACGAGGACAGTGG | ||
| QVg-F | ACAAGTCTCCGACGCCGAAG | 185 | qRT-PCR for Vg |
| QVg-R | TTGACATCGGCTTTACGGCA | ||
| Qβ-Actin-F | TCTTCCAGCCATCCTTCTTG | 173 | q(RT)-PCR for β-Actin |
| Qβ-Actin-R | CGGTGATTTCCTTCTGCATT | ||
| Vg-RNAi-F | T7-ACATCGTCAAGGCCACCAA | 451 | Vg dsRNA synthesis |
| Vg-RNAi-R | T7-TAGAGCTGGAACTAGATGAG | ||
| gfp-RNAi-F | T7-CTCGTGACCACCCTGACCTAC | 314 | dsgfp synthesis |
| gfp-RNAi-R | T7-GTTCACCTTGATGCCGTTCTT | ||
| PaL CP-GST-F | CGGGATCCATGTCGAAGCGACCCGCCG | 790 | Expression of PaL CP in E. coli |
| PaL CP-GST-R | CGGAATTCTTAATTCTGAACAGAATCA | (BamHI and EcoRI sites are underlined) | |
| TYLCV CP-GST-F | CGGGATCCATGTCGAAGCGACCAGGCGA | 793 | Expression of TYLCV CP in E. coli |
| TYLCV CP-GST-R | CGGAATTCTTAATTTGATATTGAATCAT | (BamHI and EcoRI sites are underlined) |
*
Additional primer sequences are available upon request.
†
T7, 5′-TAATACGACTCACTATAGG-3′.