Fig. 6.

Controlled oxidation of p-ethylphenol, 2. Trace i, negative control; trace ii, the reaction was sequentially catalyzed by CreHI/CreJEF (10 μM of each enzyme, 2 mM NADPH, 30 °C, 2 h) and CreD (10 μM, 30 °C, 2 h); trace iii, the reaction mixture of trace ii was supplemented with CreG (10 μM, 30 °C, 2 h) and 2 mM NAD⁺; trace iv, the reaction was first comediated by CreHI/CreJEF/CreG (10 μM of each enzyme, 30 °C, 2 h), followed by adding an additional batch of CreJEF (10 μM of each enzyme, 30 °C, 2 h) and NADPH (3 mM), and finally catalyzed by CreD (10 μM, 30 °C, 2 h). Due to distinct extinction coefficients, the peak intensity of compounds do not necessarily reflect relative amounts shown in percentage.