Skip to main content
. 2017 Jun 12;114(26):6740–6745. doi: 10.1073/pnas.1703449114

Fig. 3.

Fig. 3.

Culture of mouse ES cells (EB5) on sericin hydrogels. (A, Left) EB5 colonies formed on sericin hydrogels that were overlaid with culture medium containing blasticidin plus rhLIF or colonies were formed on sericin hydrogels incorporating polyhedron-encapsulated LIF (LIF-pol) and empty polyhedra (E-pol) or no polyhedra (Co) that were overlaid with culture medium containing blasticidin after cultivation for 7 d. (A, Right) ALP-staining positive cells are also presented. (Scale bar: 200 μm.) (B) ALP activities of EB5 on the sericin hydrogels incorporating no polyhedra after cultivation in medium containing blasticidin and rhLIF (column 1) or ALP activities of EB5 cells on sericin hydrogels incorporating polyhedron-encapsulated LIF (column 2), empty polyhedra (column 3), or no polyhedra (column 4) after cultivation for 7 d in medium containing blasticidin. Data are means ± SD (n = 3 independent samples). *P < 0.005. n.s., not significant (P > 0.05).