Fig. S5.
PTP inhibition does not affect the extent of mitochondrial depolarization induced by mitoChR2(SSFO) opening. Typical traces of single HeLa cells expressing the mitoChR2(SSFO)-YFP variant with or without the addition of the PTP inhibitor CsA (1 µM), void vector-transfected cells, or cells expressing nonfunctional mitoChR2(Tr). The cells were initially illuminated for 10 s with blue light and then kept in the dark for 1 min (black bar) followed by TMRM excitation. The bar graph represents the mean (±SEM; n ≥ 23 cells per condition) of F/Fmax changes measured 5 min after the blue light pulse, as described in Fig. 4C. NS, not significant.