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. 2017 Jun 12;114(26):E5197–E5206. doi: 10.1073/pnas.1705312114

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Fig. 6. — GK5 associates with GK through their respective FGGY_N domains. (A) HEK293T cells were transfected with either FLAG-tagged GK isoform 1 (GK-v1) or isoform 2 (GK-v2) and either HA-tagged GK5-v1 or GK5-v2. Cell lysates were immunoprecipitated using anti-FLAG M2 agarose and immunoblotted with antibodies against HA and FLAG. (B) HEK293T cells were transfected with FLAG-tagged full-length or truncated forms of GK5-v2 and HA-tagged GK-v2. The domain structures of GK5-v2 and GK are shown. The locations of the residues used in the truncated constructs are indicated below the domain structures. Cell lysates were immunoprecipitated using anti-FLAG M2 agarose and immunoblotted with indicated antibodies. (C) HEK293T cells were transfected with either the FLAG-tagged FGGY_N or FGGY_C domain of GK and either HA-tagged GK5-v1 or GK5-v2. Cell lysates were immunoprecipitated using anti-FLAG M2 agarose and immunoblotted with the indicated antibodies.