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GK5 kinase activity is necessary for normal hair growth. Mice with a kinase-inactivating point mutation of GK5 (D280N) were generated by CRISPR-Cas9 gene targeting. (A) Skin lysates from wild-type and Gk5D280N/D280N mice were immunoblotted using the indicated antibodies. GAPDH and HSP90 were the loading controls. (B) Image of wild-type and Gk5D280N/D280N mice at 6 wk of age.
