Fig. 4.

Interactions among OsGGPPS1/OsGRP heterodimer, OsGGR, and OsLIL3. (A) Y2H assay of OsGGPPS1 and OsGGR interaction. OsGGPPS1 and OsGGR were fused with the DNA binding domain (BD) of pGBK-T7 and the activation domain (AD) of pGAD-T7, respectively. Tenfold serial dilutions of yeast cells expressing both fusion proteins were spotted on nonselective (−LW) medium and selective medium supplied with X-α-Gal (−LWAH+X-α-Gal). (B and C) BiFC detection of the interactions of OsGGPPS1 (B) or OsGRP (C) with OsGGR and OsLIL3. (D) BiFC detection of the interaction between OsLIL3 and OsGGR. (E and F) BiFC assays of the colocalization of OsGGPPS1 homodimer with OsLIL3 (E) and OsGGR (F). (G and H) BiFC assays of the colocalization of OsGGPPS1/OsGRP heterodimer with OsLIL3 (G) and OsGGR (H). (Scale bars, 5 μm.)