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State-specific NOEs can be obtained for either the relaxed state or retracted state. Representative strips of NOESY spectra from (A) HN of K6 in the 3D 15N-edited 3D NOESY spectrum, (B) Hβ of I44 in the 13C-edited 3D NOESY spectrum, and (C) Hα of V70 in the 13C-edited 3D NOESY spectrum. Note that these atoms have different chemical shifts and also different NOE cross-peaks between the two states.
