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. 2017 Jun 13;114(26):6770–6775. doi: 10.1073/pnas.1705718114

Fig. S13.

Fig. S13.

Statistical analysis of smFRET data. Here we use AIC and BIC criteria (57) to identify the number of conformational states that best reproduce the observed smFRET data for (A and B) K63-diUb and (C and D) pK63-diUb at pH 7.4 and 6.4. The smFRET data were obtained with the fluorophores attached to the C terminus of the proximal Ub and the N25C site of the distal Ub in K63-diUb (or pK63-diUb). Besides the AIC and BIC parameters, the overall correlation coefficient R2 was also assessed, which is defined as 1 − RSS/TSS (RSS, sum of the square of residuals; TSS, total sum of the squares). Penalized for additional fitting parameters, both normalized AIC and BIC values reach minima with a three-state model for each system analyzed here.