Fig. 3.
The effect of early fractalkine release on SCs and endothelial cells. (A) Combined RECA (red), and DAPI (blue) staining. (B) Combined S100 (green) and DAPI (blue) staining. (C) Combined S100 (green), RECA (red), and DAPI (blue) staining. (D) Quantitative analysis of S100+ staining of SCs at the distal end of the conduit (P = 0.0243, two-tailed t test). (E) Quantitative analysis of RECA+ staining of endothelial cells at the distal end of the conduit (P = 0.0016, two-tailed t test) (n = 5). The fractalkine-treated scaffold significantly enhanced both SC infiltration and endothelial cell presence inside the nerve conduit in comparison with the IL-4–treated scaffold 4 wk after implantation. FKN, fractalkine.