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. 2005 Feb 9;5:2. doi: 10.1186/1475-2867-5-2

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Copyright © 2005 Mannechez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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K562wt ¹H-NMR spectra: effects of Triton X-100 and sphingomyelinase treatments. A: non-treated cells; B: cells fixed with PFA; C: cells fixed with PFA 4% in triton-X100 1%; D: cells fixed with PFA 4% incubated in triton-X100 1% and with 0.5 units sphingomyelinase. For peak assignment, see figure 4. Peaks at 3.6 ppm after SMase treatment arise from enzyme working buffer.