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. 2017 Jul 3;12(7):e0180213. doi: 10.1371/journal.pone.0180213

Fig 1. Isoflurane enhances macrophage phagocytosis of apoptotic PMNs.

Fig 1

BMDMs, alveolar macrophages and neutrophils (PMNs) were isolated from mice. Macrophages were labelled with CellTracker™ Green and incubated with ultraviolet-induced, CellTracker™ Red-labelled apoptotic PMNs (A, B) at 1:10 ratio for 2 h. The cells were then mounted on a slide and analyzed by fluorescence microscopy. (A) Effects of isoflurane on the phagocytosis of apoptotic PMNs by BMDMs. BMDMs were treated with isoflurane for 1 h at different concentrations (0, 0.5, 1.0 or 2.0 MAC). Left, representative fluorescent images showing macrophages engulfing apoptotic PMNs. Scale bars, 10 μm; right, phagocytic index based on the fluorescent images. (B) Effects of isoflurane on the phagocytosis of apoptotic PMNs in alveolar macrophages (AMs). AMs were treated with 1.0 MAC isoflurane for 1 h. Left, representative fluorescent images showing macrophages engulfing apoptotic PMNs Scale bars, 10 μm; right, phagocytic index based on the fluorescent images. Data are mean±SEM of three independent experiments. *P < 0.05 vs. control groups (no isoflurane).