Table 3.
Sequence coverage and peptide masses of Genotropin®
| Sequence Coverage: 86% | ||||||
| Matched peptides shown in Bold | ||||||
| 1 | FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT | |||||
| 51 | SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS | |||||
| 101 | LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFKQTYSKFDTNS | |||||
| 151 | HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F | |||||
| Start – End | Observed | Mr(expt) | Mr(calc) | Delta | Miss | Sequence |
| 1–8 | 930.61 | 929.60 | 929.53 | 0.07 | 0 | FPTIPLSR |
| 1–16 | 1906.97 | 1905.96 | 1906.01 | - 0.05 | 1 | FPTIPLSRLFDNAMLR Oxidation (M) |
| 9–16 | 979.51 | 978.50 | 978.50 | 0.01 | 0 | LFDNAMLR |
| 17–38 | 2706.34 | 2705.33 | 2705.32 | 0.00 | 1 | AHRLHQLAFDTYQEFEEAYIPK |
| 20–38 | 2342.12 | 2341.11 | 2341.13 | - 0.02 | 0 | LHQLAFDTYQEFEEAYIPK |
| 20–41 | 2727.35 | 2726.34 | 2726.32 | 0.02 | 1 | LHQLAFDTYQEFEEAYIPKEQK |
| 39–64 | 3058.51 | 3057.50 | 3057.45 | 0.05 | 1 | EQKYSFLQNPQTSLCFSESIPTPSNR |
| 42–64 | 2673.26 | 2672.26 | 2672.25 | 0.00 | 0 | YSFLQNPQTSLCFSESIPTPSNR |
| 42–70 | 3416.79 | 3415.78 | 3415.60 | 0.18 | 1 | YSFLQNPQTSLCFSESIPTPSNREETQQK |
| 65–77 | 1587.80 | 1586.79 | 1586.83 | - 0.03 | 1 | EETQQKSNLELLR |
| 78–94 | 2055.19 | 2054.18 | 2054.19 | - 0.01 | 0 | ISLLLIQSWLEPVQFLR |
| 95–115 | 2262.09 | 2261.08 | 2261.12 | - 0.04 | 0 | SVFANSLVYGASDSNVYDLLK |
| 95–127 | 3621.02 | 3620.02 | 3619.77 | 0.25 | 1 | SVFANSLVYGASDSNVYDLLKDLEEGIQTLMGR Oxidation (M) |
| 116–127 | 1377.62 | 1376.62 | 1376.66 | - 0.05 | 0 | DLEEGIQTLMGR Oxidation (M) |
| 141–158 | 2097.01 | 2096.00 | 2095.98 | 0.02 | 1 | QTYSKFDTNSHNDDALLK |
| 146–158 | 1489.66 | 1488.65 | 1488.68 | - 0.03 | 0 | FDTNSHNDDALLK |
| 146–167 | 2676.26 | 2675.25 | 2675.24 | 0.01 | 1 | FDTNSHNDDALLKNYGLLYCFR |
| 159–167 | 1205.56 | 1204.55 | 1204.57 | - 0.02 | 0 | NYGLLYCFR |
| 159–168 | 1333.64 | 1332.63 | 1332.66 | - 0.03 | 1 | NYGLLYCFRK |
| 169–178 | 1253.58 | 1252.57 | 1252.61 | - 0.04 | 1 | DMDKVETFLR |
| 169–178 | 1269.57 | 1268.56 | 1268.61 | - 0.04 | 1 | DMDKVETFLR Oxidation (M) |
Sequence coverage of 86% and 21 matching peptides of Genotropin® to human growth hormone could be detected using MALDI MS/ MS-MS data.