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Phenotype analysis by sedimentation assay. PCGFP and PCTcPFR2 trypanosomes were induced (+Tet) with tetracycline for 48 h or non-induced (-Tet) and grown in cuvettes. Non-motile cells do not swim and sediment to the bottom of the cuvette, leading to a reduction in optical density that can be monitored over time. The PCTcPFR2 cells show an almost perfect functional complementation phenotype, as induced and non-induced cells had virtually identical sedimentation curves.
