Table 2.

Silencing effciencies after transfection of various dsRNAs.

Cell line	TbPFR2 silencing in the new flagellum (% bi-flagellated cells)
	dsRNA transfected
	TbPFR2 CDS	TbPFR2 UTRs MIX
WT	88 % (n = 53)	62 % (n = 147)
TbPFR2tag	68 % (n = 113)	2 % (n = 51)
TbPFR2tag-ΔHLA	78 % (n = 100)	72 % (n = 100)

WT, TbPFR2tag and TbPFR2tag-ΔHLA trypanosomes were transfected with GFP, TbPFR2 CDS or TbPFR2 UTRs MIX dsRNAs and bi-nucleated/bi-flagellated cells were counted for the status of their new flagellum. The data presented in this table correspond to the immunofluorescence experiment described in Figure 3 (see text for details).