Fig 7. Int^pTN3-promoted low sequence specificity reactions on archaeal sequences.

Int^pTN3 catalyzes inversion on linear DNA substrates between archaeal gene pairs separated by a Kanamycin resistance determinant. White arrowheads refer to original fragments and black arrowheads indicate inversions products. A. Inversion between two identical copies of tRNA^leu gene GQS_t10759 from T. sp. 4557. B. Inversion between tRNA^Gly genes BD01_1557 and BD01_1976 from T. nautili. C. Inversion between chemotaxis genes BD01_1166 and BD01_1584 from T. nautili. Int^pTN3 concentration multipliers refer to the standard assay described in Materials and Methods. The detailed DNA sequences involved in these reactions are illustrated in S8 Fig.