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. 2017 Jun 19;13(6):e1006861. doi: 10.1371/journal.pgen.1006861

Fig 5. Detailed analysis of the siRNA coverage along the CG15098 gene.

Fig 5

A set of four biological replicates was used to determine siRNA coverage along the gene. (A) Overall, the siRNA coverage was astonishingly reproducible. This diagram shows an overlay of two independent sequencing experiments (experiment 1 colored in red and black, experiment 2 colored in orange and gray), the traces indicate the position of the 5’-end for sense-oriented siRNAs and of the 3’-end for antisense-oriented siRNAs. The read coverage thus extends 21 nt downstream of the indicated position. A close-up view of the two traces is shown in the inset. (B) The reads covering individual exons, introns and the corresponding junctions were normalized to the size of the respective feature, then the average ± siRNA.