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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 Apr 5;108:354–361. doi: 10.1016/j.freeradbiomed.2017.04.001

Figure 5. DPEN + Cu enhances cancer cell clonogenic cell killing when combined with ionizing radiation (IR) or Carboplatin.

Figure 5

(A) H292 and MB231 cancer cells were treated with 100 μM DPEN + 15 μM CuSO4 for a total of 3 hours, with exposure to 2 Gy IR after one hour of drug treatment. Catalase protected the cancer cells from the enhanced cell killing of the combination of IR + DPEN. *-Significantly different than IR alone control; p< 0.05; n= 3. ε Significantly different than DPEN alone. a Significantly different than IR + DPEN. (B) H292 cells were treated with DPEN + CuSO4 and the indicated concentrations of carboplatin for 24 hours followed by clonogenic cell survival analysis. Survival data were normalized to the toxicity of DPEN + CuSO4 alone. *Significantly different than untreated control. (C) HBEpC (n = 2 experiments plated into at least 6 cloning dishes per treatment group), H292 (n = 3 experiments plated into at least 9 cloning dishes per treatment group), and H1299 (n = 2 experiments plated into at least 6 cloning dishes per treatment group) were treated with 10 μM carboplatin for 24 hours with DPEN added for the last 3 hours, followed by clonogenic assay. CuSO4 was present in all dishes at the beginning of treatment. ** Significantly different than either drug alone. p<0.05, Errors represent ± 1 SEM.