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. 2017 Jul 3;12(7):e0180297. doi: 10.1371/journal.pone.0180297

Fig 4. Bazedoxifene-mediated inhibition is reversed by excess of IL-6 treatment or expression of constitutively active STAT3-C.

Fig 4

A, Colony formation assay was conducted in RH30 and RD cells as described in materials and methods. Cells were treated with Bazedoxifene (20 μM) for 6 hours, and then cultured with or without of IL-6, IFN-γ or IL-4 respectively for 30 minutes. Then, cells were reseeded and cultured for 2–3 weeks to grow clones. B, After treatment with Bazedoxifene (20 μM) for 6 hours, RH30 cells were stimulated with IL-6 (50 ng/ml) for 30 minutes. STAT3 phosphorylation was determined by Western blot analysis. C, RH30 cells were transfected with STAT3-C, and stable clones were selected. Selected clone cells were reseeded and treated with Bazedoxifene at indicated concentration for 48 hours. Cell viability was detected by MTT assay. Data are shown as means ±SD, *, P < 0.05; **, P < 0.01; ***, P < 0.001.