Figure 5. Surface expression of the β2 and γ2 subunits of γ-aminobutyric acid (GABA)A receptors increases after single-session and decreases after multiple-session extinction training.
(A) Experimental design. (B) Surface expression of the β2 GABAA receptor subunit and cadherin in the naïve, post-Cond, post-Ext1 and post-Ext3 groups. Surface expression levels of the β2 GABAA receptor subunit after behavioral training (post-Cond, 69.76 ± 9.260%, n = 4 from 12 rats; post-Ext1, 120.2 ± 10.25%, n = 4 from 12 rats; post-Ext3, 80.07 ± 5.919%, n = 4 from 12 rats; χ2 = 10.37, p=0.0030, *p<0.05 for post-Ext1 vs. post-Cond and post-Ext3, Kruskal-Wallis test followed by Dunn’s test) and cadherin (post-Cond, 103.2 ± 1.164%, n = 4 from 12 rats; post-Ext1, 100.1 ± 7.018%, n = 4 from 12 rats; post-Ext3, 103.7 ± 7.733%, n = 4 from 12 rats;χ2 = 1.164, p=0.7887, Kruskal-Wallis test) were expressed as a percentage of the expression in naïve controls. (C) Surface expression of the γ2 GABAA receptor subunit and cadherin in the naïve, post-Cond, post-Ext1 and post-Ext3 groups. Surface expression levels of the γ2 GABAA receptor subunit after behavioral training (post-Cond, 84.87 ± 5.364%, n = 4 from 12 rats; post-Ext1, 117.8 ± 10.09%, n = 4 from 12 rats; post-Ext3, 80.04 ± 9.232%, n = 4 from 12 rats; χ2 = 8.149, p=0.0253, *p<0.05 for post-Ext1 vs. post-Cond and post-Ext3, Kruskal-Wallis test followed by Dunn’s test) and cadherin (post-Cond, 97.10 ± 3.576%, n = 4 from 12 rats; post-Ext1, 99.38 ± 9.383%, n = 4 from 12 rats; post-Ext3, 103.4 ± 3.761%, n = 4 from 12 rats; Kruskal-Wallis test, χ2 = 0.6045, p=0.9109) levels were expressed as a percentage of the levels in the naïve controls.