Figure 4. Between genotype/within genotype differences in the amount of time experimental mice spent with partner mice in the partition assay. Between genotype comparison.

(a) Male Magel2 knockout mice (solid line) showed no difference in time spent with either baseline or novel partners when compared to male wildtype counterparts (dash line) (2-way ANOVA). (b) Female Magel2 knockout mice (solid line) spent significantly more time with baseline partner mice, and no difference in time with novel partners, compared to female wildtype mice (dash line) (2-way ANOVA). Within genotype comparison: (c,d) Both male and female wildtype mice (black bars) show significant difference between each partner pairing (experimental mouse and baseline partner/novel partner/original baseline partner). Both male and female Magel2 knockout mice (grey bars) show no significant difference between partner pairings with baseline partners and novel partner mice (unpaired t-test). Female Magel2 knockout mice show a significant difference between novel partner pairings and original partner pairings (unpaired t-test). n=20 per genotype per sex. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.