Figure 6.

Ca²⁺ uptake capacity of brain nonsynaptic mitochondria isolated from the striatum of FVB/NJ (blue traces) and YAC128 (red traces) mice. Mitochondrial Ca²⁺ uptake capacity was measured in the standard incubation medium supplemented with the Complex I substrates pyruvate (3 mM) and malate (1 mM) (A), or the Complex II substrate succinate plus glutamate (both in 3 mM) (B). In C, in addition to succinate plus glutamate, the incubation medium was supplemented with 0.1% BSA (free from fatty acids). In all experiments, the incubation medium was also supplemented with 100μM ADP and 1μM oligomycin (Chalmers and Nicholls, 2003). Where indicated, 10μM Ca²⁺ pulses (delivered as CaCl₂) were applied to mitochondria until mitochondria were unable to accumulate additional Ca²⁺ and released previously accumulated Ca²⁺. In D, the pooled group results demonstrating Ca²⁺ uptake capacity of striatal nonsynaptic mitochondria from FVB/NJ and YAC128 mice. Data are mean ± SEM, n = 4.