Figure 7.

Ca²⁺ uptake capacity of brain synaptic mitochondria isolated from the striatum of FVB/NJ (blue traces) and YAC128 (red traces) mice. Mitochondrial Ca²⁺ uptake capacity was measured in the standard incubation medium supplemented with the Complex II substrate succinate (3 mM) plus glutamate (3 mM) in the absence (A) or presence of 0.1% BSA (B). In all experiments, the incubation medium was also supplemented with 100μM ADP and 1μM oligomycin (Chalmers and Nicholls ,2003). Where indicated, 10μM Ca²⁺ pulses (delivered as CaCl₂) were applied to mitochondria until mitochondria were unable to accumulate additional Ca²⁺ and released previously accumulated Ca²⁺. In C, the pooled group results showing Ca²⁺ uptake capacity of striatal synaptic mitochondria from FVB/NJ and YAC128 mice. Data are mean ± SEM, *p<0.05 comparing mitochondria from YAC128 and FVB/NJ mice in the presence of 0.1% BSA, n = 5.